Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.
Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.
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|Alternate Names||3,3',5,5'-Tetrabromophenolsulfonphthalein; Bromophenol Blue (BPB); Bromphenol Blue Sultone Form|
|Application Notes||Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.|
|Base Catalog Number||805732|
|Beilstein Registry Number||61698|
|Melting Point||273 °C (523 °F) (Lit.)|
|Pack Size||10 g|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|pH||Visual Transition Interval Yellow (pH3.0) to Purple (pH4.6)|
|Solubility||Soluble in ethanol (0.1% w/v-clear yellow solution), methanol (40mg/4mL), EGME, water, benzene.|
|Vapor Pressure||Extremely low (Lit.)|