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Phytohemagglutin-leukoagglutinin

Product Description

Phytohemagglutin-Leukoagglutinin

Application Notes

Phytohemagglutin is very valuable in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Reconstitute with water to a final concentration of 25 mg/mL (0.2 mL water for the 5 mg size). This concentration of the lectin has been used for iontophoresis and neuron transport.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Mitogenic activity | Cell Biology | Cell Signaling and Neuroscience

Specifications

SKU	02151886-CF
Alternate Names	PHA-L; Phaseolus vulgaris agglutinin; PHA; PHA-P, Lectin from Phaseolus vulgaris
Application Notes	Phytohemagglutin is very valuable in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Reconstitute with water to a final concentration of 25 mg/mL (0.2 mL water for the 5 mg size). This concentration of the lectin has been used for iontophoresis and neuron transport.
Base Catalog Number	151886
Biochemical Physiological Actions	PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
CAS #	Not yet assigned
Format	Lyophilized powder
Hazard Statements	H317
Personal Protective Equipment	Eyeshields, Gloves, Respirator filter
Source	Phaseolus vulgaris (Red Kidney Bean)
Specificity	Carbohydrate Specificity: PHA-L recognizes a complex branched chain oligosaccharide structure.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.