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Lysozyme, ≥9000 Sugar U/mg solid, from chicken eggwhite

Lysozyme, ≥9000 Sugar U/mg solid, from chicken eggwhite

$39.20

Key features and details

Lysozyme

SKU: 02100834-CF

Synonyms
Muramidase; Mucopeptide N-acetylmuramoylhydrolase
CAS Number:
9001-63-2
Molecular Weight:
2235.597 g/mol
EC Number:
235-747-3
MDL Number:
MFCD00131557
Product Description

Lysozyme

Application Notes

Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Germinative agent | Extracting group specific antigen

Specifications
SKU 02100834-CF
Alternate Names Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Application Notes Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts.
Base Catalog Number 100834
Biochemical Physiological Actions Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
CAS # 9001-63-2
EC Number 235-747-3
Extinction Coefficient 26.4 (Lit.)
Format Crystalline powder
Hazard Statements H317
Isoelectric Point 11.0(Lit.)
Molecular Weight 2235.597 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
RTECS Number OL5987000
Source Chicken eggwhite
Specific Activity ≥9000 Sugar U/mg solid
Unit Definition One unit will cause a decrease in OD450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

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