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Lysozyme, ≥20,000 U/mg solid

Lysozyme, ≥20,000 U/mg solid

$32.65

Key features and details

  • Lysozyme from chicken egg white Active over a broad pH range (6.0 to 9.0) and ionic strength (0.02 to 0.1 M) Enzyme breaks down the cell walls of bacteria

SKU: 02100831-CF

Synonyms
Muramidase; Mucopeptide N-acetylmuramoylhydrolase
CAS Number:
9001-63-2
Molecular Weight:
2235.597 g/mol
EC Number:
232-620-4
MDL Number:
MFCD00131557
Product Description

Lysozyme

Application Notes

Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell Lysis

Specifications
SKU 02100831-CF
Alternate Names Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Application Notes Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Base Catalog Number 100831
Biochemical Physiological Actions Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
CAS # 9001-63-2
EC Number 232-620-4
Extinction Coefficient 26.4 (280 nm) (Lit.)
Format lyophilized, Salt-free, 3X Crystallized, Albumin-Free
Isoelectric Point pH 11.0 (lit)
Molecular Weight 2235.597 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
RTECS Number OL5987000
Source Chicken eggwhite
Specific Activity ≥20,000 u/mg solid
Unit Definition One unit will cause a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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