OFFER EXTENDED! Get Your $20 Credit For Every $100 Purchase, Free 2021 Wall Calendar & Free Shipping*. Use promo code: MPHOLIDAY at the checkout. Offer ends Jan. 15, 2021. T&C apply.
In Stock
Ribonuclease A (bovine)
Specifications
SKU | 02101076-CF |
Alternate Names | Pancreatic Ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′ pyrimidinooligonucleotidohydrolase |
Application Notes | Ribonuclease A, and to a greater extent its oligomers and some homologs (such as onconase from frogs), have cytotoxic and cytostatic effects, particularly on cancer cells.This attribute has led to the development of onconase as a cancer therapeutic. |
Base Catalog Number | 101076 |
Biochemical Physiological Actions | Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2'-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. |
CAS # | 9001-99-4 |
EC Number | 232-646-6 |
Extinction Coefficient | 7.3 (280 nm) (Lit.) |
Isoelectric Point | pH 9.45 (Lit.) |
Molecular Weight | 13700 (based on Amino acid sequence) |
Personal Protective Equipment | Eyeshields, Gloves, Repirator Filter |
pH | Optimum pH: 7.0 - 7.5 |
RTECS Number | RF0760000 |
Solubility | Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution. |
Source | Bovine Pancreas |
Specific Activity | ≥50 Kunitz u/mg |
Specificity | The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains. |
Unit Definition | That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity at 25 °C and pH 5.0. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Citations
Documents
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Datasheet
Datasheet
Material Safety Datasheet
Material Safety Datasheet
en_AE
Material Safety Datasheet
de_AT
Material Safety Datasheet
en_AU
Material Safety Datasheet
fr_BE
Material Safety Datasheet
en_CA
Material Safety Datasheet
cn_ZH
Material Safety Datasheet
zh_CN
Material Safety Datasheet
de_DE
Material Safety Datasheet
es_ES
Material Safety Datasheet
en_EU
Material Safety Datasheet
fi_FI
Material Safety Datasheet
fr_FR
Material Safety Datasheet
en_GB
Material Safety Datasheet
tw_ZH
Material Safety Datasheet
en_ID
Material Safety Datasheet
en_IE
Material Safety Datasheet
en_IN
Material Safety Datasheet
ja_JP
Material Safety Datasheet
de_LI
Material Safety Datasheet
de_LU
Material Safety Datasheet
en_MT
Material Safety Datasheet
es_MX
Material Safety Datasheet
no_NO
Material Safety Datasheet
pt_PT
Material Safety Datasheet
sv_SE
Material Safety Datasheet
en_US