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Pectinase
Product Description
Pectinase
Application Notes
Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used in plant protoplast preparation to digest cell wall
Specifications
SKU | 02198979-CF |
Alternate Names | Polygalacturonase; EC 3.2.1.15; Poly-[1,4-alpha-D-galacturonide] glycanohydrolase; Macerozyme R-10 |
Application Notes | Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. |
Base Catalog Number | 198979 |
Biochemical Physiological Actions | Pectinase causes random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. |
CAS # | 9032-75-1 |
EC Number | 232-885-6 |
Format | Powder |
Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
Source | Asperigillus niger |
Specific Activity | >1000 u/gm |
Typical Working Concentration | A concentration of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase), 24 °C to 37 °C for periods of 1 to 16 hours will yield good results for protoplasting. |
Unit Definition | One unit will liberate one µmole of galacturonic acid from polygalacturonicacid per minute at pH 4.0 at 25 °C. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Citations
Documents
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
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