Pectinase

Application Notes

Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Used in plant protoplast preparation to digest cell wall

SKU	02198979-CF
Alternate Names	Polygalacturonase; EC 3.2.1.15; Poly-[1,4-alpha-D-galacturonide] glycanohydrolase; Macerozyme R-10
Application Notes	Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Base Catalog Number	198979
Biochemical Physiological Actions	Pectinase causes random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
CAS #	9032-75-1
EC Number	232-885-6
Format	Powder
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Source	Asperigillus niger
Specific Activity	>1000 u/gm
Typical Working Concentration	A concentration of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase), 24 °C to 37 °C for periods of 1 to 16 hours will yield good results for protoplasting.
Unit Definition	One unit will liberate one µmole of galacturonic acid from polygalacturonicacid per minute at pH 4.0 at 25 °C.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.