OFFER EXTENDED! Get Your $20 Credit For Every $100 Purchase, Free 2021 Wall Calendar & Free Shipping*. Use promo code: MPHOLIDAY at the checkout. Offer ends Jan. 15, 2021. T&C apply.
Papain, technical grade, >1750 USP U/mg
Papain
Application Notes
Papain is widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have also been isolated from adult salamander retina with papain. Papain used to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Used for dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons. It is also used to produce Fab fragments of antibodies. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
For production of Fab fragments of antibodies | Used for cell dissociation
SKU | 02102566-CF |
Alternate Names | Papayotin; Vegetable Pepsin; Caroid; Vermizym; Papainase |
Application Notes | Papain is widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have also been isolated from adult salamander retina with papain. Papain used to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Used for dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons. It is also used to produce Fab fragments of antibodies. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. |
Base Catalog Number | 102566 |
Biochemical Physiological Actions | Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. It is also hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. |
CAS # | 9001-73-4 |
EC Number | 232-627-2 |
Extinction Coefficient | 25.0 (Lit.) |
Format | Powder |
Grade | Technical Grade |
Hazard Statements | H315-H319-H334-H335 |
Isoelectric Point | pH 9.6 (Lit.) |
Molecular Weight | 226.236 g/mol |
Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
RTECS Number | RU4950000 |
Safety Symbol | GHS07, GHS08 |
Specific Activity | >1750 USP u/mg |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |