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Collagenase type II, >125 U/mg solid

Collagenase type II, >125 U/mg solid

$49.90

Key features and details

  • From Clostridium histolyticum
  • Protease which cleaves collagen
  • For use in the preparation of isolated cell suspensions

SKU: 02100502-CF

Synonyms
Clostridiopeptidase A
CAS Number:
2593923
EC Number:
232-582-9
MDL Number:
MFCD00130830
Product Description

Collagenase Type Ii

Application Notes

Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Release of cells from animal tissues

Specifications
SKU 02100502-CF
Alternate Names Clostridiopeptidase A
Application Notes Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.
Base Catalog Number 100502
CAS # 2593923
EC Number 232-582-9
Format Lyophilized powder
Hazard Statements H315-H319-H334-H335
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Safety Symbol GHS07, GHS08
Source Clostridium histolyticum
Specific Activity >125 u/mg solid
Specificity Clostridial collagenase I or clostridiopeptidase A degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence -Pro-Y-Gly-Pro- where Y is most frequently a neutral amino acid. This bond in synthetic peptide substrates may also be split.
Unit Definition One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
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