Collagenase, Type I
Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.
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|Alternate Names||Clostridiopeptidase A|
|Application Notes||Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.|
|Base Catalog Number||195109|
|Biochemical Physiological Actions||Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N',N'-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.|
|Personal Protective Equipment||Dust mask , Eyeshields, Faceshields, Gloves|
|Protein or Enzyme Type||Proteins, Enzymes & Peptides|
|Safety Symbol||GHS07, GHS08|
|Specific Activity||>125 u/mg|
|Unit Definition||One unit will liberate peptides from collagen equivalent in ninhydrin color to 1.0 μmole of L-leucine in 5 hr at pH 7.4 and 37 °C in presence of calcium ions. Contains clostripain, neutral protease and trypsin activities.|