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Collagenase, type I, >125 U/mg

Collagenase, type I, >125 U/mg

$39.20

Key features and details

  • From Clostridium histolyticum
  • Protease which cleaves collagen
  • For use in the preparation of isolated cell suspensions

SKU: 02195109-CF

Synonyms
Clostridiopeptidase A
CAS Number:
2593923
EC Number:
232-582-9
MDL Number:
MFCD00130830
Product Description

Collagenase, Type I

Application Notes

Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Therapeutic uses

Specifications
SKU 02195109-CF
Alternate Names Clostridiopeptidase A
Application Notes Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.
Base Catalog Number 195109
Biochemical Physiological Actions Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N',N'-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.
CAS # 2593923
EC Number 232-582-9
Format Lyophilized powder
Personal Protective Equipment Dust mask , Eyeshields, Faceshields, Gloves
Protein or Enzyme Type Proteins, Enzymes & Peptides
Safety Symbol GHS07, GHS08
Source Clostridium histolyticum
Specific Activity >125 u/mg
Unit Definition One unit will liberate peptides from collagen equivalent in ninhydrin color to 1.0 μmole of L-leucine in 5 hr at pH 7.4 and 37 °C in presence of calcium ions. Contains clostripain, neutral protease and trypsin activities.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Datasheet

Datasheet