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Product Description
Alkaline Phosphatase
Application Notes
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Conjugation | Protein Labelling
Specifications
| SKU | 02199817-CF |
| Alternate Names | Orthophosphoric Monoester Phosphohydrolase |
| Application Notes | Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. |
| Base Catalog Number | 199817 |
| Biochemical Physiological Actions | Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters. |
| CAS # | 9001-78-9 |
| EC Number | 232-631-4 |
| Format | Ammonium Sulfate Suspension |
| Molecular Weight | 496.569 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | E. Coli |
| Specific Activity | ≥30 u/mg protein , Activity- ~1000 u/mL |
| Unit Definition | One unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 10.4, 37 °C. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
![MP COA [199817] lot ID: 1421J](https://www.mpbio.com/static/version20220119103500Z/frontend/Mpbio/default/en_US/images/document.png "MP COA [199817] lot ID: 1421J")