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Alkaline phosphatase

Alkaline phosphatase

$270.85

Key features and details

  • From E. coli

SKU: 02199817-CF

Synonyms
Orthophosphoric Monoester Phosphohydrolase
CAS Number:
9001-78-9
Molecular Weight:
496.569 g/mol
EC Number:
232-631-4
MDL Number:
MFCD00131849
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Product Description

Alkaline Phosphatase

Application Notes

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Conjugation | Protein Labelling

Specifications
SKU 02199817-CF
Alternate Names Orthophosphoric Monoester Phosphohydrolase
Application Notes Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.
Base Catalog Number 199817
Biochemical Physiological Actions Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
CAS # 9001-78-9
EC Number 232-631-4
Format Ammonium Sulfate Suspension
Molecular Weight 496.569 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Source E. Coli
Specific Activity ≥30 u/mg protein , Activity- ~1000 u/mL
Unit Definition One unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 10.4, 37 °C.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificate of Analysis

Certificate of Analysis
MP COA [199817] lot ID: 1421J MP COA [199817] lot ID: 1421J
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Certificate of Analysis
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Material Safety Datasheet

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