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Diethyl pyrocarbonate

Diethyl pyrocarbonate

$63.85

Key features and details

Diethyl Pyrocarbonate

SKU: 02150902-CF

Synonyms
Diethyl Oxydiformate; Dicarbonic Acid Diethyl Ester; DEPC; Ethoxyformic acid anhydride; Diethyl dicarbonate; DEP; Pyrocarbonic acid diethyl ester; Oxydiformic acid diethyl ester
CAS Number:
1609-47-8
Molecular Formula:
C6H10O5
Molecular Weight:
162.141 g/mol
Beilstein Registry Number:
637031
EC Number:
216-542-8
MDL Number:
MFCD00009106
Product Description

Diethyl Pyrocarbonate

Application Notes

DEPC is effective as a nuclease inhibitor.Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl Pyrocarbonate is used to modify proteins and nucleic acids. Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Specifications
SKU 02150902-CF
Alternate Names Diethyl Oxydiformate; Dicarbonic Acid Diethyl Ester; DEPC; Ethoxyformic acid anhydride; Diethyl dicarbonate; DEP; Pyrocarbonic acid diethyl ester; Oxydiformic acid diethyl ester
Application Notes DEPC is effective as a nuclease inhibitor.Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl Pyrocarbonate is used to modify proteins and nucleic acids. Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.
Base Catalog Number 150902
Beilstein Registry Number 637031
Biochemical Physiological Actions Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles..
CAS # 1609-47-8
EC Number 216-542-8
Hazard Statements H302-H315-H319-H335
Melting Point < 25 °C
Molecular Formula C6H10O5
Molecular Weight 162.141 g/mol
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge respirator filter
Refractive Index n20/D 1.398(lit.)
RTECS Number LQ9350000
Safety Symbol GHS07
Typical Working Concentration For Treatment of water to inhibit RNase: Add 1 ml DEPC to 1 liter of water. Let sit overnight. Autoclave.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificate of Analysis (COA)