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Product Description
An aminonucleoside antibiotic that is toxic to both prokaryotic and eukaryotic cells. It causes premature chain termination in protein synthesis and is widely used in cell culture as a selection agent.
Application Notes
Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Inhibitor of aminopeptidase and enkephalinase.
Specifications
| SKU | 02194539-CF |
| Alternate Names | Stylomycin ; 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride |
| Application Notes | Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase. |
| Base Catalog Number | 194539 |
| Beilstein Registry Number | 3853613 |
| Biochemical Physiological Actions | Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days. |
| CAS # | 58-58-2 |
| EC Number | 200-387-8 |
| Format | Powder |
| Grade | Cell Culture Grade |
| Hazard Statements | H302 |
| Molecular Formula | C22H29N7O5-2HCl |
| Molecular Weight | 544.40 |
| Personal Protective Equipment | Dust mask , Eyeshields, Gloves |
| RTECS Number | AU7355000 |
| Safety Symbol | GHS07 |
| Solubility | Soluble (NTP, 1992) |
| Typical Working Concentration | recommended working concentration in eukaryotic cell culture is 1-10 µg/mL |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | λ max (0.1N HCl) 268 ± 2 nm |
Documents
![MP COA [194539] lot ID: 1195A](https://www.mpbio.com/static/version20220125073033Z/frontend/Mpbio/default/en_US/images/document.png "MP COA [194539] lot ID: 1195A")