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Isis DNA polymerase (1 U/µl)

Isis DNA polymerase (1 U/µl)

Synonyms High Fidelity Polymerase |Proofreading DNA Polymerase

EC Number: 232-741-2

SKU 11EPSIS100-CF
$128.00
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Description

Product Description

Isis™ DNA Polymerase is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase.

Application Notes

Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR

Specifications
SKU 11EPSIS100-CF
Alternate Names High Fidelity Polymerase |Proofreading DNA Polymerase
Application Notes Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences
Base Catalog Number EPSIS100
Brand Isis™
Components 100 U Isis™ DNA Pol at 1 U/µL |Isis™ buffer 10x C (1ml) with MgSO4 |1 mL MgSO4 at 30 mM
Concentration 1 U/µl
EC Number 232-741-2
Format Kit
Grade Molecular Biology Grade
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Protein or Enzyme Type Molecular Biology Enzymes
References (1) Flaman, J.M., et al. (1994) Nucl. Acids Res. 15, 3259-3260 |(2) Flaman, J.M., et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967 |(3) Gueguen, Y. et al., (2001) Eur. J. Biochem. 268, 1-10 |(4) Gueguen, Y. et al., (2002) FEMS Microbiology Letters. 217, 89-94
Unit Definition One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.