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Ethidium bromide, 25 g

Ethidium bromide, 25 g

Synonyms 2,7-Diamino-10-ethyl-9-phenyl- phenanthridinium bromide; Homidium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; EtBr; Dromilac

CAS Number: 1239-45-8 Molecular Formula: C21H20N3Br Molecular Weight: 394.3

Beilstein Registry Number: 3642536 EC Number: 214-984-6 MDL Number: MFCD00011724

Packaging size: 25 g

SKU 0219020225
$428.10
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Specifications
SKU 0219020225
Alternate Names 2,7-Diamino-10-ethyl-9-phenyl- phenanthridinium bromide; Homidium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; EtBr; Dromilac
Application Notes Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. Confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands.
Base Catalog Number 19020225
Beilstein Registry Number 3642536
Biochemical Physiological Actions Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
CAS # 1239-45-8
EC Number 214-984-6
Hazard Statements H302-H330-H341
Melting Point 260-262 °C (dec.) (Lit.)
Molecular Formula C21H20N3Br
Molecular Weight 394.3
Pack Size 25 g
Personal Protective Equipment Eyeshields, Faceshields, full-face particle respirator, Gloves, respirator cartridge, respirator filter
RTECS Number SF7950000
Safety Symbol GHS06, GHS08
Solubility Soluble in water (10mg/mL), Slightly soluble in ethanol; partially soluble in chloroform.
Typical Working Concentration Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/mL and visualized immediately after electrophoresis.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance λmax = 475 nm; 520 nm (methanol), 480 nm (water); 515 (glycerol); 520 nm (acetone); 532 nm (ethanol); 535 (DMSO) and 540 nm (pyridine) (Lit.)