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Creatinine amidohydrolase, 150 units/mg solid
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
|Application Notes||This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.|
|Base Catalog Number||153491|
|Biochemical Physiological Actions||Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the ß-strands and the N termini of the main a-helices. These zinc ions indicate the location of the active site.|
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator|
|Specific Activity||150 units/mg solid|
|Unit Definition||One unit causes the formation of one µmole of creatinine-picrate per minute at pH 7.5 at 37°C.|