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Agarose, high-gelling, molecular biology grade, 100 g

Agarose, high-gelling, molecular biology grade, 100 g

$784.30

Key features and details

High gelling temperature agarose (gel point 42 °C) for nucleic acid electrophoresis.

SKU: 04800667

Synonyms
3,6-Anhydro-α-L-galacto-β-D-galactan; Sepharose; FastLane agarose; Indubiose A4; NuSieve GTG; Odigose; Seakem
CAS Number:
9012-36-6
EC Number:
232-731-8
MDL Number:
MFCD00081294
Packaging size:
100 g
Product Description

Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae.

Application Notes

As a gelling agent, agarose is used: 1.) to separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic); 2.) To demonstrate cross reaction in IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied; 3.) to make gel plates or overlays for cells in tissue culture; 4.) To form a gel matrix (either beaded and/or crosslinked) which can be used in chromatographic separations.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Specifications
SKU 04800667
Alternate Names 3,6-Anhydro-α-L-galacto-β-D-galactan; Sepharose; FastLane agarose; Indubiose A4; NuSieve GTG; Odigose; Seakem
Application Notes As a gelling agent, agarose is used: 1.) to separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic); 2.) To demonstrate cross reaction in IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied; 3.) to make gel plates or overlays for cells in tissue culture; 4.) To form a gel matrix (either beaded and/or crosslinked) which can be used in chromatographic separations.
Base Catalog Number 800667
CAS # 9012-36-6
EC Number 232-731-8
Gel Temperature ~42 °C
Melting Point 85.7 °C (Lit.)
Pack Size 100 g
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pH pH (1.5% aq soln): 6.86 pH in gel (1.5% aq soln): 6.03 (Lit.)
Purity ≥97%
Solubility Soluble in 1.5 g plus 100 mL of H2O - Clear to Very Slightly Hazy.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Datasheet

Datasheet

Material Safety Datasheet

Material Safety Datasheet
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