Anti-human fiberonectin, goat antiserum, 60.2mg/ml, 2 mL

Product Description

Product is the lyophilized powder of goat antiserum to human fibronectin and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is recommended for detection of Fibronectin of mouse, rat and human origin by Western Blotting, immunoprecipitation [1-2 ug per 100-500 ug of total protein (1ml ofcell lysate)], immunofluorescence, immunohistochemistry(including paraffin-embedded sections) and solid phase ELISA.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Precipitating antibody | Immunofluorescence | Immunohistochemistry

Specifications

SKU	0855125
Antibody Type	Polyclonal Antibody
Application Notes	This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is recommended for detection of Fibronectin of mouse, rat and human origin by Western Blotting, immunoprecipitation [1-2 ug per 100-500 ug of total protein (1ml ofcell lysate)], immunofluorescence, immunohistochemistry(including paraffin-embedded sections) and solid phase ELISA.
Base Catalog Number	55125
Concentration	Total Protein: 60.2 mg/mL
Host	Goat
Pack Size	2 ml
Preparation Method	The antiserum is prepared by hyperimmunizing goats with highly purified human fibronectin; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 um filter, vialed and lyophilized.
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Sterilization of Solutions	Filtered through a 0.22 um filter
Typical Working Concentration	Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.