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The Fragile X Syndrome is identified by an ampiflication of the FMR1 (CGG)n repeat region. The Fragile X CHEMITM probe is a digoxigenin labeled DNA probe (pFxa1NHE) which can be used in conjunction with the Sure Blot® CHEMITM Kit (Cat. No. 11S4250KIT) to detect (CGG) repeats in the FMR-1 gene. The same results are obtained as when using the standard P32-labeled probes, but without radioactivity. The amplification of the FMR-1 gene is detected on Southern blots by an increase in the band sizes. Also provided is a ready-to-use mixture of unlabeled and digoxigenin-labeled Lambda DNA/Hind III Molecular Weight Markers for convenient visualization on both ethidium bromide-stained agarose gels and Southern blots.
Fragile X CHEMI™ DNA Probe is used to detect Fragile X Syndrome, which is identified by an amplification of the FMR1 (CGG)n repeat region.
Detection of Fragile X Syndrome
|Alternate Names||Digioxigenin labeled probe|
|Application Notes||Fragile X CHEMI™ DNA Probe is used to detect Fragile X Syndrome, which is identified by an amplification of the FMR1 (CGG)n repeat region.|
|Base Catalog Number||S1120DIG|
|Pack Size||10 Tests|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. Use in Fume Hood|