o-Nitrophenyl β-D-galactopyranoside is a colorimetric substrate for β-galactosidase.
o-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase. It is also used as a cell culture component for molecular genetics.
Cell culture component for molecular genetics | Substrate for β-galactosidase
|Alternate Names||o-Nitrophenyl-β-D -galactoside; 2-Nitrophenyl -D-galactopyranoside; ONPG; 2-Nitrophenyl β-D-galactopyranoside|
|Application Notes||o-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase. It is also used as a cell culture component for molecular genetics.|
|Base Catalog Number||19403005|
|Beilstein Registry Number||92207|
|Biochemical Physiological Actions||β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.|
|Melting Point||190 - 202°C|
|Pack Size||5 g|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|UV Visible Absorbance||UV/VIS: λ max (water): 262 ± 3 nm|