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Taq DNA Polymerase Enzyme
Taq DNA Polymerase is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences. Other application include RT-PCR, DNA sequencing and DNA labeling.
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Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |Routine PCR
|Alternate Names||Taq polymerase |Routine PCR Enzyme|
|Application Notes||Taq DNA Polymerase is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences. Other application include RT-PCR, DNA sequencing and DNA labeling.|
|Base Catalog Number||EPTQA001|
|Biochemical Physiological Actions||Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.|
|Components||Taq DNA Pol at 5 U/µL |10x Incubation Buffer (1ml) with MgSO4 |10x Incubation Buffer (1ml) without MgSO4 |1 mL MgSO4 at 25 mM|
|Grade||Molecular Biology Grade|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Protein or Enzyme Type||Molecular Biology Enzymes|
|Unit Definition||One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.|