Isis™ DNA Polymerase is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase.
Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences
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Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR
|Alternate Names||High Fidelity Polymerase |Proofreading DNA Polymerase|
|Application Notes||Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences|
|Base Catalog Number||EPSIS100|
|Components||100 U Isis™ DNA Pol at 1 U/µL |Isis™ buffer 10x C (1ml) with MgSO4 |1 mL MgSO4 at 30 mM|
|Grade||Molecular Biology Grade|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Protein or Enzyme Type||Molecular Biology Enzymes|
|References||(1) Flaman, J.M., et al. (1994) Nucl. Acids Res. 15, 3259-3260 |(2) Flaman, J.M., et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967 |(3) Gueguen, Y. et al., (2001) Eur. J. Biochem. 268, 1-10 |(4) Gueguen, Y. et al., (2002) FEMS Microbiology Letters. 217, 89-94|
|Unit Definition||One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.|