The FastDNA Spin Kit for Soil is designed to isolate bacterial, fungal, plant, and animal genomic DNA from soil and other environmental samples. Soil is processed by a FastPrep® instrument and Lysing Matrix E tubes designed to efficiently lyse all microorganisms. The released DNA is purified by a silica-based spin filter method and is suitable for PCR analysis and other downstream applications. Complete lysis of all organisms, including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi is achieved within seconds using a FastPrep instrument and Lysing Matrix E tubes.
Cited in over 6000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors
Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
|Alternate Names||FastDNA Soil Kit|
|Application Notes||Cited in over 6000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors|
|Base Catalog Number||6560200|
|Components||50 x 2.0 mL tubes of Lysing Matrix E, 60 mL Sodium Phosphate Buffer, 8 mL MT Buffer, 25 mL of PPS Solution, 66 mL Binding Matrix, 50 Spin Modules, 50 Catch Tubes, 12 mL Concentrated SEWS-M, 20 mL DES, 200 µl BBS Gel Loading Dye|
|FastPrep Instruments Models||ConeFlex Legacy (allows all FP24 adapters on FP96), CoolPrep (24x2 mL), HiPrep (48x2 mL), QuickFlex (96x2 mL), QuickPrep (24x2 mL)|
|Hardness||Ceramic Spheres, Vickers 800; Silica Spheres, Knoop 515; Glass Bead, Knoop 515|
|Personal Protective Equipment||If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (Labcoat and long-sleeves), and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices.|
|pH||Reagent: SEWS-M pH Range: 6.4-6.8|
|Sample Type||Seeds, Spores, Bacteria (Gram +/-), Animal Tissues, Feces, Food, Fungi, Plants, Roots, Soil, Wastewater, Yeast|
|Sample Volume||up to 500 mg per prep|
|Specific Gravity||Ceramic Spheres, 4; Silica Spheres, 2.5; Glass Bead, 2.5|
Certificate of Analysis
Material Safety Datasheet
FDA validated molecular method to detect C. cayetanensis in vegetable food samples.
Skim milk drastically improves the efficacy of DNA extraction from andisol, a volcanic ash soil.
Detection of Kudoa septempunctata feces 18S ribosomal DNA in patient fecal samples from novel food-borne outbreaks caused by consumption of raw olive flounder.
Optimization of 16S rRNA gut microbiota profiling of extremely low birth weight infants.
Microbes on building materials - evaluation of DNA extraction protocols as common basis for molecular analysis.