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EMM without Dextrose is a minimal defined medium for the growth of S. pombe, containing buffers, salts, vitamins and trace minerals. EMM minimal defined medium is thiamine-free nmt1 promoter studies in Schizosaccharomyces pombe.
EMM w/o Dextrose is used for the growth of Schizosaccharomyces pombe.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
|Alternate Names||Edinburgh's Minimal Medium w/o Dextrose |S.pombe growth medium|
|Application Notes||EMM w/o Dextrose is used for the growth of Schizosaccharomyces pombe.|
|Base Catalog Number||4110112|
|Formulation Details||Contents Per Liter: 3 g potassium hydrogen phthalate (14.7mM); 2.2g Na2HPO4 (15.5mM); 5g NH4Cl (93.5mM); 1.05g MgCl2.6H2O (5.2mM); 14.7mg CaCl2.2H2O (99.8µM); 1g KCl (13.4mM); 0.04g Na2SO4 (0.28mM); 1mg pantothenic acid (4.2µM); 10mg nicotinic acid (81.2µM); 10mg myo-inositol (55.5µM); 10µg biotin (0.82µM); 0.5 mg boric acid (8.9µM); 0.4mg MnSO4 (2.4µM); 0.4mg ZnSO4.7H2O (1.4µM); 0.2mg FeCl2.6H2O (0.7µM); 40µg molybdic acid (0.25µM); 0.1mg KI (0.6µM); 40µg CuSO4.5H2O (0.016µM); 1mg citric acid (4.76µM).|
|Grade||Molecular Biology Certified™|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range: 5.4 - 6.4 at 25 °C|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in EMM Broth formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Solubility||Soluble in water (12.35 g/L).|
|Sterilization of Solutions||Autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).|