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YES is a nutritionally rich medium for the growth of S. pombe, containing dextrose, and yeast extract. Dextrose is the energy source. Yeast extract provides carbon, nitrogen, minerals, vitamins, trace ingredients and other essential growth nutrients which stimulate the growth of yeasts.
EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
|Alternate Names||Edinburgh's Minimal Medium |S.pombe growth medium|
|Application Notes||EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.|
|Base Catalog Number||4101522|
|Formulation Details||Contents Per Liter: 5 g Yeast Extract-Y; 30 g Dextrose; 1X Supplements (50 mg each ADE; HIS; LEU; LYS; URA). Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range: 5.5 - 6.5 at 25 °C|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in YES Broth formulations. Chosen reagents are combined in the proper proportions according to the YES formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Solubility||Soluble in water (35 g/L).|