YNB is available with or without ammonium sulfate. YNB is composed of salts, vitamins and trace elements. Some component dropouts are available. To make your own synthetic defined yeast media, start with the YNB of your choice, add a carbon source, the appropriate amino acids, and agar if necessary.
YNB w/ Ammonium Sulfate is a component of synthetic defined yeast media.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Yeast Nitrogen Base|
|Application Notes||YNB w/ Ammonium Sulfate is a component of synthetic defined yeast media.|
|Base Catalog Number||4027512|
|Formulation Details||Components Per liter medium-Nitrogen Sources-Ammonium Sulfate: 5000 mg; Vitamins-Biotin: 0.002 mg; Calcium Pantothenate: 0.4 mg; Folic Acid: 0.002 mg; Inositol: 2 mg; Niacin: 0.4 mg; P-Aminobenzoic Acid :0.2 mg; Pyridoxine Hydrochloride: 0.4 mg; Riboflavin: 0.2 mg; Thiamine Hydrochloride: 0.4 mg; Trace Elements- Boric Acid: 0.5 mg Copper Sulfate: 0.04 mg; Potassium Iodide: 0.1 mg; Ferric Chloride: 0.2 mg; Manganese Sulfate: 0.4 mg; Sodium Molybdate: 0.2 mg Zinc Sulfate: 0.4 mg; Salts- Magnesium Sulfate: 500 mg; Sodium Chloride: 100 mg; Potassium Phosphate; monobasic: 1000 mg; Calcium Chloride: 100 mg|
|Grade||Molecular Biology Certified™|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||4.7 + 0.5|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YNB formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution.|
|Solubility||Soluble in water(6.7 g/L).|
|Typical Working Concentration||6.7g/liter|