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LB-Agar medium is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in a variety of formats for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium. Agar is already included in this convenient formulation.
LB Agar Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. LB Agar Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
|Alternate Names||Luria Bertani Medium with Agar |Luria Bertani Broth with Agar |Luria Broth Base Miller Formulation with Agar|
|Application Notes||LB Agar Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. LB Agar Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.|
|Base Catalog Number||3002265|
|Formulation Details||Typically contains 10 g Tryptone; 5 g Yeast Extract; 10 g NaCl; 15 g Agar-B. Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.5 ± 0.3 at 35 °C.(Lit.)|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Agar Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Agar formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Solubility||Soluble in water (20 g/500 mL)|
|Typical Working Concentration||40 g/L|