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2-Mercaptoethanol

2-Mercaptoethanol

$17.65

Key features and details

2-Mercaptoethanol

SKU: 02194834-CF

Synonyms
2-Thioethanol; 2-Hydroxyethylmercaptan; 2-Hydroxy-1-Ethanethiol; Monothioethyleneglycol; β-Mercaptoethanol; BME; Thioethylene glycol; 2-Hydroxyethyl Mercaptan; 1-ethane-2-thiol; 2-ME; 2-thioethanol
CAS Number:
60-24-2
Molecular Formula:
C2H6OS
Molecular Weight:
78.129 g/mol
Beilstein Registry Number:
773648
EC Number:
200-464-6
MDL Number:
MFCD00004890
Product Description

2-Mercaptoethanol

Application Notes

2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Purification | Reducing agent

Specifications
SKU 02194834-CF
Alternate Names 2-Thioethanol; 2-Hydroxyethylmercaptan; 2-Hydroxy-1-Ethanethiol; Monothioethyleneglycol; β-Mercaptoethanol; BME; Thioethylene glycol; 2-Hydroxyethyl Mercaptan; 1-ethane-2-thiol; 2-ME; 2-thioethanol
Application Notes 2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Auto Ignition 295°C
Base Catalog Number 194834
Beilstein Registry Number 773648
Boiling Point 315 to 316° F at 742 mm Hg (decomposes) (NTP, 1992)
CAS # 60-24-2
Density 1.1143 at 68° F (NTP, 1992)
EC Number 200-464-6
Flash Point 165° F (NFPA, 2010)
Format Liquid
Grade Molecular Biology Grade
Hazard Statements H301-H310 + H330-H315-H317-H318-H410
Melting Point -148° F (NTP, 1992)
Molecular Formula C2H6OS
Molecular Weight 78.129 g/mol
Personal Protective Equipment Faceshields, Full-face respirator, Gloves, Goggles, Multi-purpose combination respirator cartridge
Purity ≥98%
Refractive Index n20/D 1.500(lit.)
RTECS Number KL5600000
Safety Symbol GHS05, GHS06, GHS09
Solubility greater than or equal to 100 mg/mL at 68° F (NTP, 1992)
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Vapor Density 2.69 (NTP, 1992) (Relative to Air)
Vapor Pressure 1 mm Hg at 68° F (NTP, 1992)
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