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Bis-Tris, cell culture reagent, ≥99%

Bis-Tris, cell culture reagent, ≥99%

$47.60

Key features and details

Bis-Tris

SKU: 02194546-CF

Synonyms
2,2-Bis-(hydroxymethyl)-2,2´,2´´- nitrilotriethanol; bis(2-hydroxymethyl)imino-tris (hydroxymethyl)methane; 2-bis(2-Hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propandiol
CAS Number:
6976-37-0
Molecular Formula:
C8H19NO5
Molecular Weight:
209.242 g/mol
Beilstein Registry Number:
2205275
EC Number:
230-237-7
MDL Number:
MFCD00002853
Product Description

Bis-Tris

Application Notes

Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Used in electrophoresis buffer

Specifications
SKU 02194546-CF
Alternate Names 2,2-Bis-(hydroxymethyl)-2,2´,2´´- nitrilotriethanol; bis(2-hydroxymethyl)imino-tris (hydroxymethyl)methane; 2-bis(2-Hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propandiol
Application Notes Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization.
Base Catalog Number 194546
Beilstein Registry Number 2205275
CAS # 6976-37-0
EC Number 230-237-7
Extinction Coefficient EM (water): 259 nm = 10; 277 nm = 10 (Lit.)
Format Powder
Grade Cell Culture Grade
Hazard Statements H315-H319-H335
Molecular Formula C8H19NO5
Molecular Weight 209.242 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Gloves
pKa 6.5 (25 °C)(Lit.)
Purity ≥99%
Safety Symbol GHS07
Sterilization of Solutions For sterilization of aqueous solutions, MP Biomedicals recommends sterile filtration with a 0.2 µm filter; however, the product is typically stable for autoclaving.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
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