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Coomassie Brilliant Blue R-250

Coomassie Brilliant Blue R-250

$27.70

Key features and details

Coomassie Brilliant Blue R-250 stain for use in protein gel staining.

SKU: 02190682-CF

Synonyms
Brilliant Blue R; Acid Blue 83; C.I. 42660; Brilliant indocyanin 6B, Coomassie Brilliant Blue R
CAS Number:
6104-59-2
Molecular Formula:
C45H44N3NaO7S2
Molecular Weight:
825.971 g/mol
Beilstein Registry Number:
5718025
EC Number:
228-060-5
MDL Number:
MFCD00041762
Product Description

Coomassie® Brilliant Blue R-250

Application Notes

Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates. Coomassie Blue R-250 is additionally used in the Bradford Assay to quantify the concentration of protein in a solution, such as cell lysates. A fast acting, sensitive dye which can be used on SDS gels, IEF gels, and standard gels.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Hematology | Staining

Specifications
SKU 02190682-CF
Alternate Names Brilliant Blue R; Acid Blue 83; C.I. 42660; Brilliant indocyanin 6B, Coomassie Brilliant Blue R
Application Notes Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates. Coomassie Blue R-250 is additionally used in the Bradford Assay to quantify the concentration of protein in a solution, such as cell lysates. A fast acting, sensitive dye which can be used on SDS gels, IEF gels, and standard gels.
Base Catalog Number 190682
Beilstein Registry Number 5718025
Biochemical Physiological Actions The dye molecule binds to proteins to form a protein-dye complex. This complex stabilizes the negatively charged anionic form of the dye producing the blue color, even under acidic conditions.
CAS # 6104-59-2
Color Index 42660
EC Number 228-060-5
Format Powder
Molecular Formula C45H44N3NaO7S2
Molecular Weight 825.971 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Typical Working Concentration Prepare in distilled, deionized water: 50% (v/v) methanol, 0.05% (w/v) Coomassie Brilliant Blue R-250, 10% acetic acid, 40% Water. Dissolve the Coomassie Brilliant Blue R in methanol before adding acetic acid and water (filtration is not generally needed). Solution can be stored for 6 months. If precipitate is observed following prolonged storage, filter to obtain a homogeneous solution.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 585 ± 5 nm (ethanol)
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