Alkaline phosphatase
Alkaline Phosphatase
Application Notes
Alkaline phosphatase is used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations | Used in immunoblotting; ELISA′s; and other antibody based detection assays
| SKU | 02190680-CF |
| Alternate Names | EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP |
| Application Notes | Alkaline phosphatase is used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays. |
| Base Catalog Number | 190680 |
| Biochemical Physiological Actions | Catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase. |
| CAS # | 9001-78-9 |
| EC Number | 232-631-4 |
| Format | Powder |
| Isoelectric Point | 5.7(Lit.) |
| Molecular Weight | 496.569 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | Calf Intestine |
| Specific Activity | ≥1.0 u/mg |
| Unit Definition | That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 25 °C and pH 9.6. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [190680] lot ID: 1127B](https://www.mpbio.com/static/version20210119154001Z/frontend/Mpbio/default/en_US/images/pdf.png "MP COA [190680] lot ID: 1127B")