In Stock

Chlortetracycline hydrochloride, >900 Units/mg

Product Description

Chlortetracycline Hydrochloride

Application Notes

Cell-permeable fluorescent probe for Ca2+ for measuring calcium flux in endoplasmic reticulum and other intracellular stores in situ.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antibacterial | Antibiotics

Specifications

SKU	02190327-CF
Alternate Names	Aureomycin; Chlorotetracycline hydrochloride; 7-Chlorotetracycline hydrochloride; aureocycline; auxeomycin; biomycin; 7-chlorotetracycline
Application Notes	Cell-permeable fluorescent probe for Ca2+ for measuring calcium flux in endoplasmic reticulum and other intracellular stores in situ.
Base Catalog Number	190327
Beilstein Registry Number	3858364
Biochemical Physiological Actions	Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-positive. Less active against Gram-negative bacterial than tetracycline. Mode of Resistance: Loss of cell wall permeability.
CAS #	64-72-2
EC Number	200-591-7
Format	Powder
Hazard Statements	H315-H319-H335
Molecular Formula	C22H23ClN2O8 • HCl
Molecular Weight	515.34 g/mol
Personal Protective Equipment	Dust mask , Eyeshields, Gloves
pKa	3.3, 7.4 and 9.3 at 25 °C.(Lit.)
Preparation Method	To prepare Stock Solutions: Add the appropriate amount of powder to water while mixing (not the reverse), as adding water to the powders may result in insoluble mixtures that cannot be filtered without losing potency. If desired, the pH may be adjusted, but a basal media with greater than 2 g/L sodium bicarbonate can easily absorb up to 2 % of an acidic antibiotic solution; therefore, an antibiotic solution with an acidic pH would not influence the pH of the media. After ensuring that the resultant solution is transparent, sterile filter and store in small aliquots at appropriate temperature until use. Prior to use of the media, aseptically add the appropriate amount of antibiotic to the basal media in order to obtain the required working concentration. Mix well and sterile filter if asepsis is believed to have been compromised.
RTECS Number	QI7800000
Safety Symbol	GHS07
Specific Activity	>900 u/mg
Sterilization of Solutions	Solutions should be filter sterilized. Store in small aliquots at appropriate temperature until use.
Typical Working Concentration	For Gram Positive Bacteria & Gram Negative Bacteria : 10 mg/L
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.