Ampicillin Trihydrate

Application Notes

Ampicillin Trihydrate is commonly used to select for ampicillin resistance in mutated and transformed cells. It is also described for the use of inhancing luminol chemiluminescence.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antibiotic

SKU	02190147-CF
Alternate Names	Aminobenzylpenicillin trihydrate; alpha-Aminobenzylpenicillin trihydrate ;D[-]-α-Aminobenzylpenicillin; 6-[D(-)-α-Aminophenylacetamido]penicillanic acid
Application Notes	Ampicillin Trihydrate is commonly used to select for ampicillin resistance in mutated and transformed cells. It is also described for the use of inhancing luminol chemiluminescence.
Base Catalog Number	190147
Beilstein Registry Number	5399534
Biochemical Physiological Actions	A β-lactam antibiotic with an amino group side chain attached to the penicillin structure. Penicillin derivative that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane. Bactericidal only to growing Escherichia coli. Mode of resistance: Cleavage of β-lactam ring of ampicillin by β-lactamase. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
CAS #	7177-48-2
EC Number	200-709-7
Format	Powder
Hazard Statements	H315-H317-H319-H334-H335
Melting Point	388 to 392° F (decomposes) (NTP, 1992)
Molecular Formula	C16H19N3O4S·3H2O
Molecular Weight	403.45 g/mol
Personal Protective Equipment	Dust mask, Eyeshields, Faceshields, Gloves
pKa	At 25 °C ,pKa = 2.5 (-COOH),pKa = 7.3 (-NH2) (Lit.)
RTECS Number	XH8425000
Safety Symbol	GHS07, GHS08
Solubility	1 to 10 mg/mL at 70° F (NTP, 1992)
Sterilization of Solutions	Autoclaving solutions of ampicillin will destroy activity. Solutions can be sterilized by filtration (0.22 um filter) and stored frozen (-20 °C or below). Frozen solutions can be stored for up to 3 months.
Typical Working Concentration	100 mg/liter for both gram positive and gram negative bacteria. It is typically stable in media at 37 °C for approximately 3 days.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.