Alkaline Phosphatase

Application Notes

Alkaline phosphatase is used for removing phosphate monoester to prevent self ligation. It can be also be used as a label for enzyme immunoassays.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

SKU	02159347-CF
Alternate Names	Orthophosphoric Monoester Phosphohydrolase; Alkaline phosphomonoesterase
Application Notes	Alkaline phosphatase is used for removing phosphate monoester to prevent self ligation. It can be also be used as a label for enzyme immunoassays.
Base Catalog Number	159347
CAS #	9001-78-9
EC Number	232-631-4
Format	Lyophilized powder
Hazard Statements	H317
Isoelectric Point	5.7(Lit.)
Molecular Weight	496.569 g/mol
Source	Calf Intestine
Unit Definition	One unit liberates one µmole of p-nitrophenol per minute at 37 °C, pH 10.15 in Tris buffer. The enzyme activity is determined by measuring the increase in optical density at 405 nm resulting from the hydrolysis of p-nitrophenyl phosphate.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.