Hygromycin B Solution

Application Notes

Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is used in gene transfer experiments as a selection agent.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antibacterial and antifungal agent

SKU	02157513-CF
Alternate Names	Antihelmycin; Hygromix-8
Application Notes	Hygromycin has been used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is used in gene transfer experiments as a selection agent.
Base Catalog Number	157513
Beilstein Registry Number	6755837
Biochemical Physiological Actions	Antibacterial and antifungal. Mode of action: Inhibition of protein synthesis, it interfere with translocation of the 70S ribosome, by inducing the misreading of the m-RNA template in prokaryotes and eukaryotes. It selectively penetrates cells that have been rendered permeable by virus infection.
CAS #	31282-04-9
EC Number	250-545-5
Format	Solution
Formulation Details	Hygromycin B is provided as a caramel-colored solution in water. Not prepared under sterile conditions. If a sterile solution is desired; working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use. Do not autoclave solutions of Hygromycin B.
Hazard Statements	H301 + H311-H318-H330-H334
Molecular Formula	C20H37N3O13
Personal Protective Equipment	Eyeshields, Faceshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
pKa	7.1 and 8.8(Lit.)
Purity	≥80%
RTECS Number	WK2130000
Safety Symbol	GHS05, GHS06, GHS08
Specific Activity	~450,000 u/mL
Sterilization of Solutions	Solutions should be sterilized by means of a 0.2 micron filter. Do not autoclave solutions.
Typical Working Concentration	For use as a selective agent, the suggested concentration range is 100- 800 μg/mL
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.