Bumetanide

Application Notes

Bumetanide has been used to probe the role of prostaglandin E2 in the regulation of chloride secretion across porcine endometrial epithelial cells. A study of the effects of bumetanide application and subsequent sodium potassium chloride cotransporter (NKCC1) inhibition in cultured liver cells has indicated increased α1-smooth muscle actin expression in 2-day-cultured hepatic stellate cells. An investigation of lactacidosis treatment of suspended G6 glial cells with bumetanide included at 0.1 mM has shown diminished cell swelling due to lactacidosis.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

SKU	02154263-CF
Alternate Names	Ro 10-6338; 3-(aminosulfonyl)-5-(butylamino)- 4-phenoxybenzoic acid; 3-(butylamino)-4-phenoxy- 5-sulfamoylbenzoic acid
Application Notes	Bumetanide has been used to probe the role of prostaglandin E2 in the regulation of chloride secretion across porcine endometrial epithelial cells. A study of the effects of bumetanide application and subsequent sodium potassium chloride cotransporter (NKCC1) inhibition in cultured liver cells has indicated increased α1-smooth muscle actin expression in 2-day-cultured hepatic stellate cells. An investigation of lactacidosis treatment of suspended G6 glial cells with bumetanide included at 0.1 mM has shown diminished cell swelling due to lactacidosis.
Base Catalog Number	154263
Biochemical Physiological Actions	Inhibitor of Na+/K+/Cl- cotransporter.
CAS #	28395-03-1
EC Number	249-004-6
Format	Powder
Melting Point	230-231
Molecular Formula	C17H20N2O5S
Molecular Weight	364.416 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Purity	≥99%
RTECS Number	DG4910000
Solubility	>54.7 [ug/mL]
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.