Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP.
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|Alternate Names||PK; ATP : pyruvate 2-O-phosphotransferase; Phosphoenolpyruvate kinase|
|Application Notes||Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP.|
|Base Catalog Number||151999|
|Biochemical Physiological Actions||Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.|
|Extinction Coefficient||5.4 (280 nm) (Lit.)|
|Personal Protective Equipment||Eyeshields, Gloves, Repirator Filter|
|Specific Activity||≥200 u/mg protein|
|Unit Definition||That amount of enzyme causing the oxidation of one micromole of NADH per minute at 25 °C and pH 7.4.|