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Hyaluronidase is used for extravasation of hyperosmolar solutions. It is also used to study human umbilical cord stem cell encapsulation in calcium phosphate scaffolds for bone engineering.
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|Alternate Names||Hyaluronate 4-glycanohydrolase; hyaluronate lyase; hyaluronoglucosaminidase; E.C. 188.8.131.52|
|Application Notes||Hyaluronidase is used for extravasation of hyperosmolar solutions. It is also used to study human umbilical cord stem cell encapsulation in calcium phosphate scaffolds for bone engineering.|
|Base Catalog Number||151275|
|Biochemical Physiological Actions||These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.|
|Personal Protective Equipment||Eyeshields, Gloves,respirator filter|
|Specific Activity||≥1,000 I.U./mg material|
|Specificity||Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B); primarily to tetrasaccharide residues. Monosaccharides are not liberated.|
|Typical Working Concentration||For Hyaluronic acid measurement assay-stock solution of enzyme at one mg/mL in 0.1 M sodium phosphate buffer pH 5.3 with 0.15 M sodium chloride.|
|Unit Definition||That amount of enzyme causing the same turbidity reduction as the "International Unit" (I.U.) as compared with the international standard.|