Cephalexin Monohydrate

Application Notes

Cephalexin is a cephalosporin antibiotic used to study the effect of expression, binding, and inhibition of PBP3 and other penicillin-binding proteins (PBPs) on bacterial cell wall mucopeptide synthesis. Cephalexin and other antibiotics have been shown to inhibit p-aminohippurate uptake by the rat renal organic anion transporter 1, as expressed in Xenopus laevis oocytes. A study of CHO cells transfected with the vector for the H+-coupled peptide transporter PEPT1 has shown that cephalexin is a competitor for dipeptide transport. Cephalexin has been used to culture E. coli at 10 µg/mL for the expression of fusion proteins.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antibacterial | Antibiotics

SKU	02150585-CF
Alternate Names	7-(d-a-Aminophenylacetamido)desacetoxycephalosporanic acid; Cefalexin monohydrate; [6R-[6α,7β(R*)]]-7-[(aminophenylacetyl)amino]-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-carboxylic acid monohydrate; 7-(Dα-aminophenylacetamido) desacetoxycephalosporanic acid monohydrate
Application Notes	Cephalexin is a cephalosporin antibiotic used to study the effect of expression, binding, and inhibition of PBP3 and other penicillin-binding proteins (PBPs) on bacterial cell wall mucopeptide synthesis. Cephalexin and other antibiotics have been shown to inhibit p-aminohippurate uptake by the rat renal organic anion transporter 1, as expressed in Xenopus laevis oocytes. A study of CHO cells transfected with the vector for the H+-coupled peptide transporter PEPT1 has shown that cephalexin is a competitor for dipeptide transport. Cephalexin has been used to culture E. coli at 10 µg/mL for the expression of fusion proteins.
Base Catalog Number	150585
Beilstein Registry Number	965503
Biochemical Physiological Actions	Cephalexin disrupts the synthesis of the peptidoglycan layer of bacterial cell walls which is responsible for cell wall structural integrity. Peptidoglycan synthesis is facilitated by transpeptidases known as penicillin-binding proteins (PBPs). PBPs bind to the D-Ala-D-Ala at the end of muropeptides (peptidoglycan precursors) to crosslink the peptidoglycan. Cephalexin antibiotics mimic the D-Ala-D-Ala site, thereby competitively inhibiting PBP crosslinking of peptidoglycan.
CAS #	23325-78-2
EC Number	239-773-6
Extinction Coefficient	7.75(Lit.)
Format	Powder
Grade	USP
Hazard Statements	H317-H334
Melting Point	326.8 °C
Molecular Formula	C16H17N3O4S·H2O
Molecular Weight	365.404 g/mol
Optical Rotation	+149° to +158° (pH 4.4)
Personal Protective Equipment	Dust mask , Eyeshields, Faceshields, Gloves
pKa	5.2, 7.3(Lit.)
Potency	950 - 1010 µg/mg (anhydrous basis)
Purity	≥95%
RTECS Number	XI0350000
Safety Symbol	GHS08
Solubility	Water Solubility1.789 mg/mL as monohydrate salt (solubility in water is low at room temperature; 1 or 2 mg/mL may be dissolved readily, but higher concentrations are obtained with increasing difficulty)
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	260 - 262 nm (water)