Lactase

Application Notes

Lactase is used to screen for blue white colonies in the MCS of various plasmid vectors in Escherichia coli or other bacteria. Its primary commercial use is to break down lactose in milk to make it suitable for people with lactose intolerance.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Screen for blue white colonies | Break down lactose in milk

SKU	02100780-CF
Alternate Names	β-D-Galactoside galactohydrolase; Lactase from Aspergillus oryzae; β-Galactosidase
Application Notes	Lactase is used to screen for blue white colonies in the MCS of various plasmid vectors in Escherichia coli or other bacteria. Its primary commercial use is to break down lactose in milk to make it suitable for people with lactose intolerance.
Base Catalog Number	100780
Biochemical Physiological Actions	β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
EC Number	232-864-1
Format	Powder
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
RTECS Number	LW5840000
Source	Aspergillus oryzae
Specific Activity	Activity >5000 u/g
Unit Definition	One unit will hydrolyze 1.0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4.5 and 30 °C.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.