YT media formulations are optimized for the growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.
YT medium agar is used for growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.
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Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
|Alternate Names||Bacteriophage Media|
|Application Notes||YT medium agar is used for growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.|
|Base Catalog Number||3011231|
|Formulation Details||Contents Per Liter: 8 g Tryptone-B; 5 g Yeast Extract-B; 5 g NaCl; 15 g Agar-B|
|Grade||Molecular Biology Certified™|
|Pack Size||1 kg|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.9 ± 0.3 at 25°C.|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in YT-Broth Agar formulations. Chosen reagents are combined in the proper proportions according to the YT-Broth Agar formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 33 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Solubility||Soluble in water (33 g/L).|