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YPD (YEPD) is a nutritionally rich medium for the growth of S. cerevisiae, containing dextrose, peptone and yeast extract. Dextrose is the energy source. Yeast extract and peptone provide carbon, nitrogen, minerals, vitamins, trace ingredients and other essential growth nutrients which stimulate the growth of yeasts.
YPD Broth is used for routine growth of Saccharomyces cerevisiae for molecular biology studies.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Yeast Extract Peptone Dextrose Broth|
|Application Notes||YPD Broth is used for routine growth of Saccharomyces cerevisiae for molecular biology studies.|
|Base Catalog Number||4001036|
|Formulation Details||Contents Per Liter: 20 g Peptone Y; 10 g Yeast Extract-Y; 20 g Dextrose. Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™|
|Pack Size||1 kg|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||Acceptable pH range is 6.2 ± 0.3 at 25 °C|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YPD Broth formulations. Chosen reagents are combined in the proper proportions according to the YPD formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Solubility||Soluble in water (50 g/L).|
|Sterilization of Solutions||Combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).|
|Typical Working Concentration||50 g/L|