SOC Powder is a special medium for incubating competent cells immediately after transformation to allow expression of transferred resistance genes before exposing cells to selective conditions.
SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Medium used in recovery step of Escherichia coli competent cell transformation
|Alternate Names||Competent Cell Media for Bacteria|
|Application Notes||SOC Medium is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC maximizes the transformation efficiency of competent cells.|
|Base Catalog Number||3031012|
|Components||Contents Per Liter: 20 g Tryptone, 5 g Yeast Extract, 0.5 g NaCl, 5 g MgSO4 - 7H2O, 20 mM Glucose. Small quantities of biological buffers have been added for proper pH adjustment.|
|Formulation Details||Typically contains per liter: 20 g Tryptone-B; 5 g Yeast Extract-B; 0.5 g NaCl; 5 g MgSO4∙7H2O; 20 mM glucose. Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™|
|Pack Size||227 g|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.3 ± 0.3 at 25 °C. Please note that the pH of SOC Medium liquid increases as the temperature of the solution decreases.|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in SOC formulations. Chosen reagents are combined in the proper proportions according to the SOC formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 31 g of powder per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121°C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Solubility||Soluble in water (31 g/L).|
|Sterility||0.2 μm filtered|
|Usage Statement||Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.|