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Isis core kit 10 mM, 100 U
Product Description
ISIS CORE KIT (with 10 mM dNTPs )
Application Notes
Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR
Specifications
| SKU | 11EPSISK100 |
| Alternate Names | High Fidelity Polymerase |Proofreading DNA Polymerase |
| Application Notes | Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences |
| Base Catalog Number | EPSISK100 |
| Brand | Isis™ |
| Components | 100 U Isis™ DNA Pol at 1 U/µL |dNTPs at 10 mM each |Isis™ buffer 10xC (1mL) with MgSO4 |1 mL MgSO4 at 30 mM |dNTPs 10 mM |
| Concentration | 1 U/µl |
| EC Number | 232-741-2 |
| Format | Kit |
| Grade | Molecular Biology Grade |
| Pack Size | 100 U |
| Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Protein or Enzyme Type | Molecular Biology Enzymes |
| References | (1) Flaman, J.M., et al. (1994) Nucl. Acids Res. 15, 3259-3260 |(2) Flaman, J.M., et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967 |(3) Gueguen, Y. et al., (2001) Eur. J. Biochem. 268, 1-10 |(4) Gueguen, Y. et al., (2002) FEMS Microbiology Letters. 217, 89-94 |
| Unit Definition | One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |