IgG1 (Kappa) MOPC 21, mouse myeloma, purified, 1 mg
Product is purified mouse IgG1 (K) and buffer. Mouse IgG1 (K) is purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Application Notes
This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | ELISA | Control
| SKU | 0850327 |
| Alternate Names | Mouse IgG1/k |
| Antibody Type | Monoclonal Antibody |
| Application Notes | This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. |
| Base Catalog Number | 50327 |
| Clone Name | MOPC 21 |
| Concentration | Total Protein: 1.2 mg/mL |
| Conjugate | Unconjugated |
| Formulation Details | Purified |
| Host | Mouse |
| Isotype | IgG |
| Pack Size | 1 mg |
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge |
| Preparation Method | Mouse IgG1 (K) is purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed. |
| Purity | Affinity Purified |
| Solubility | Soluble in Water |
| Specificity | The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG1. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG1; no trace of IgG2a; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected. |
| Sterility | Filtered through a .22 µm filter |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [50327] lot ID: 00454](https://www.mpbio.com/static/version20201229105009Z/frontend/Mpbio/default/en_US/images/pdf.png "MP COA [50327] lot ID: 00454")
