IgA (Lambda2) MOPC 315, mouse myeloma, purified, 1 mL

Product Description

Mouse IgA (l2) is purified from clarified MOPC 315 mouse ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M Tris, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. It is suitable for Ouchterlony Double Diffusion. The antibody was used to detect antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Ouchterlony Double Diffusion

Specifications

SKU	0850325
Antibody Type	Monoclonal Antibody
Application Notes	This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. It is suitable for Ouchterlony Double Diffusion. The antibody was used to detect antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL.
Base Catalog Number	50325
Clone Name	MOPC 315
Concentration	1.1 mg/mL
Conjugate	Unconjugated
Formulation Details	Purified
Host	Mouse
Isotype	IgA
Pack Size	1 mL
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Preparation Method	Mouse IgA (λ2) is purified from clarified MOPC 315 mouse ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M Tris, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Purity	Affinity Purified
Solubility	Soluble in Water
Specificity	The total protein is determined using the Biuret procedure with bovine albumin as the standard and a phosphate blank. The product contains 1 mg of mouse IgA. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgA; no trace of IgG; IgM or non-immunoglobulin serum proteins is detected.
Sterility	Filtered through a .22 µm filter
Typical Working Concentration	Detection of antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL .
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.