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Anti-rat IgG (whole molecule) goat IgG fraction, peroxidase-conjugated, 2 mL

Anti-rat IgG (whole molecule) goat IgG fraction, peroxidase-conjugated, 2 mL

$396.30

Key features and details

Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to rat IgG (whole molecule) and buffer salts.

SKU: 0855770

Packaging size:
2 mL
Product Description

Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rat IgG (whole molecule) and buffer salts.

Application Notes

Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855770
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
Base Catalog Number 55770
Concentration Total Protein: 5.5 mg/mL Titer: 1:120;000
Conjugate HRP
Formulation Details Lyophilized
Host Goat
Pack Size 2 mL
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity Purified
Solubility Soluble in water
Species Reactivity Rat (Whole Molecule)
Specificity It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration SUGGESTED WORKING DILUTIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1;8,000 for Immunoblotting 1:2,000 - 1:16,000 for Elisa
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations