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Anti-human IgG Fc goat IgG fraction, peroxidase-conjugated , 2 mL

Anti-human IgG Fc goat IgG fraction, peroxidase-conjugated , 2 mL

$413.85

Key features and details

Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG Fc and buffer salts.

SKU: 0855226

Packaging size:
2 mL
Product Description

Peroxidase-conjugated goat IgG fraction to human IgG Fc is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG Fc and buffer salts.

Application Notes

Peroxidase-conjugated goat IgG fraction to human IgG Fc is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855226
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-conjugated goat IgG fraction to human IgG Fc is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals.
Base Catalog Number 55226
Concentration Total Protein: 39.0 mg/mL; Titer: 1:200;000
Conjugate HRP
Formulation Details Lyophilized
Host Goat
Immunogen Human IgG (Fc) fragment
Isotype IgG
Pack Size 2 mL
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human, IgG Fc
Typical Working Concentration ELISA (ELISA) 1:10,000; Immunohistochemistry (Frozen) (IHC (F)) 1:500; Western Blot (WB) 1:1000, 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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