Anti-mouse immunoglobulins (IgG, IgA, IgM) affinity-purified goat F(Ab')2 fragment, fluorescein-conjugated, 2 mg

Product Description

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified F(ab')₂ fragments to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications

SKU	0855526
Antibody Type	Polyclonal Antibody
Application Notes	This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number	55526
Concentration	Total Protein: 1.05 mg/mL; F/P Ratio: 5.2 mol/mol
Conjugate	FITC
Formulation Details	Purified
Host	Goat
Pack Size	2 mg
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Purity	Affinity Purified
Solubility	Soluble in water
Species Reactivity	Mouse (IgG, IgA, IgM)
Specificity	This product shows reactivity to mouse immunoglobulins (IgG;IgA;IgM); cross-reactivity to other species may exist. Antibody activity to non-immunoglobulin serum proteins is not present.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.