Fluorescein-Conjugated Goat IgG Fraction To Mouse IgG (No Cross To Rat)

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse IgG (no cross to rat) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat Anti Mouse IgG is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

SKU	0855503
Antibody Type	Polyclonal Antibody
Application Notes	Fluorescein-Conjugated Goat Anti Mouse IgG is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number	55503
Concentration	Total Protein: 14.9 mg/mL; Antibody Protein: 3.0 mg/mL
Conjugate	FITC
Formulation Details	Lyophilized
Host	Goat
Immunogen	Mouse IgG
Pack Size	2 mL
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity	Purified
Solubility	Soluble in water
Species Reactivity	Mouse (No Cross To Rat)
Specificity	This product shows reactivity to mouse IgG. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration	Anti-Mouse IgG (whole molecule)-FITC antibody is suitable for use in immunohistochemistry (1:200) and immunofluorescence (1:100).
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.