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Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human lambda light chain (bound) and buffer salts.
This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
|Antibody Type||Polyclonal Antibody|
|Application Notes||This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)|
|Base Catalog Number||55160|
|Concentration||Total Protein: 11.2 mg/mL F/P Ratio: 5.86 mol/mol|
|Pack Size||2 mL|
|Preparation Method||Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.|
|Solubility||Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|
|Species Reactivity||Human, Lambda Light Chain (Bound)|
|Specificity||This product shows reactivity to bound lambda light chains; cross-reactivity to other species may exist. Because the product is directed against the lambda chains in Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to free lambda light chains may be present. Antibody activity to kappa Fab; free kappa light chains; or non-immunoglobulin serum proteins is not present.|
|Typical Working Concentration||DILUTION RECCOMENDATIONS: 1:100 - 1:400 for Tissue Staining 1:250 - 1:1,000 for Immuno Blotting 1:1,000 - 1:4,000 for ELISA/FIA|